thermal acclimation  (MiddleBrook Pharmaceuticals)

Journal: Evolutionary Applications

Article Title: Patterns of methylation and transcriptional plasticity during thermal acclimation in a reef‐building coral

doi: 10.1111/eva.13757

Figure Lengend Snippet: (a) Experimental design showing the acclimation treatments and how samples from each acclimation treatment were distributed for the heat stress assay. *Day 0 samples in the 31°C tank never actually experienced 31°C acclimation. (b) Plot of chlorophyll a concentration redrawn from previously performed study. Samples in the 31°C (Day 11) acclimation tank have a higher chlorophyll a concentration following the 5‐h 34°C heat stress assay. (c) Prediction of the relationship between change in gene expression response to heat stress following acclimation and the change in average percent DNA methylation. The green ellipse symbolizes the hypothesized positive association between these variables. The purple ellipse symbolizes the alternative hypothesis if there is no relationship between the variables.

Article Snippet: In their 3‐week thermal acclimation experiment, which involved switching coral fragments from elevated thermal exposure to the control thermal environment at two sampling timepoints, they observed no association between shifts in gene body methylation and either reversible or irreversible shifts in gene expression (Abbott et al., ).

Techniques: Concentration Assay, Gene Expression, DNA Methylation Assay

Journal: Evolutionary Applications

Article Title: Patterns of methylation and transcriptional plasticity during thermal acclimation in a reef‐building coral

doi: 10.1111/eva.13757

Figure Lengend Snippet: (a) Plot of mean percent methylation of amplified and dampened transcripts. *** p < 0.001. (b) Plot of the log 10 of mean expression of amplified and dampened transcripts. **** p < 0.0001. (c) Scatterplot of acclimation‐associated gene expression (i.e. Wald statistic) and DNA methylation changes. The oval represents the 99% CI. Legend colors depict genes with significant upregulation and downregulation following acclimation.

Article Snippet: In their 3‐week thermal acclimation experiment, which involved switching coral fragments from elevated thermal exposure to the control thermal environment at two sampling timepoints, they observed no association between shifts in gene body methylation and either reversible or irreversible shifts in gene expression (Abbott et al., ).

Techniques: Methylation, Amplification, Expressing, Gene Expression, DNA Methylation Assay